Protein Purification Reagents and Kits
Medchemexpress LLC Protein G Agarose | 50 ML
Protein G is a bacterial cell wall protein isolated from group G Streptococci that binds to most mammalian IgGs primarily through Fc regions. Recombinant Protein G, with its albumin and cell surface binding sites eliminated to reduce nonspecific binding, is coupled with 4% highly cross-linked agarose in this product. It effectively purifies mammalian monoclonal and polyclonal antibodies, including human IgG3 and rat IgG2a.
- Composition: 4% cross-linked agarose
- Degree of substitution: 2 mg recombinant protein G/mL settled resin
- Bead diameter: 45-165 µm
- Binding capacity: 20 mg human IgG/mL settled resin
- Storage: 50% slurry in 1× PBS with 20% ethanol; stable for at least 2 years at 2-8°C; do not dry or freeze.
Biotium CD62L (L-Selectin)(CD62L/1588), Biotin conjugate, 0.1mg/mL
Selectins, also designated CD62 antigens, comprise a family of carbohydrate-binding proteins involved in mediating cellular interactions with leukocytes. L-Selectin (also designated LECAM-1 or CD62L) is expressed on the majority of B and naive T cells and on most monocytes, neutrophils and eosinophils. L-Selectin interacts with specific carbohydrates expressed by activated endothelial cells. P-Selectin (also designated GMP-140 or CD62P), expressed on activated platelets and endothelial cells, and E-Selectin (also designated ELMA-1 or CD62E), expressed on endothelial cells, exhibit overlapping ligand specificities. Both recognize sialyl-Le (x) as a ligand and bind to specific carbohydrates on neutrophils and monocytes. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecti
Bulldog Bio Inc GFPTRAP AGAROSE 0.5ML
Reagent; Molecular Biology; GFP-Trap, Coupled to Agarose, for Immuno-Pulldown of GFP Fusions, 20 Reactions, 0.5ml
Cell Signaling Technology SimpleChIP Human 28S rDNA Repeat Primers 500 µl
SimpleChIP Human 28S rDNA Repeat Primers 500 µl
Invent Biotechnologies Inc Minute™ High Fidelity ER Isolation Kit for Liver Tissue - spin column-based (20 Preps)
Endoplasmic reticulum (ER) is a major membranous structure that functionally connects nuclear membrane and plasma membrane. Liver is an ER-rich organ and has been used for isolation of ER for decades. Traditional methods for isolating ER are based on density-gradient ultracentrifugation. The protocol requires a large amount of starting material and the methods are tedious and time-consuming and contain significant cross-contamination. Currently, all commercial kits for ER isolation are based on methods developed in the 1970s. Unlike any other ER isolation kit on the market, this kit employs a patented spin-column-based technology that is simple and rapid and requires only a small amount of tissue. This kit can differentially precipitate native ER (mainly rough ER) from frozen liver tissues without using a Dounce homogenizer or ultracentrifugation.
Revvity Health Sciences Inc Copper HIS-Tag YSI SPA Beads, 125 mg
Copper HIS-Tag YSI SPA Beads, 125 mg
Immunology Consultants anti-HA Tag Antibody (Chicken) - Affinity Purified
This 0.1 mg format of the affinity‑purified anti‑HA tag antibody provides the same high‑performance detection as the full‑size vial in a smaller, cost‑efficient quantity. Produced in chicken, this antibody delivers excellent sensitivity and low background across ELISA, Western blotting, immunoprecipitation, and imaging workflows. Its affinity‑purified preparation ensures consistent binding and strong signal‑to‑noise ratios, making it ideal for pilot studies, small‑scale assays, or labs requiring limited volumes of high‑quality HA tag detection reagent.
Immunology Consultants anti-DYKDDDDK (Flag) Antibody Chicken - Affinity Purified
This affinity‑purified anti‑DYKDDDDK (FLAG) antibody provides a high‑specificity reagent for detecting FLAG‑tagged recombinant proteins across a wide range of expression systems. Produced in chicken and supplied at 1 mg, this antibody offers strong binding, low background, and excellent compatibility with ELISA, Western blotting, immunoprecipitation, and other FLAG‑tag detection workflows. Its affinity‑purified format ensures consistent performance and high signal‑to‑noise ratios, making it ideal for researchers requiring reliable detection of FLAG‑tagged constructs in protein expression, purification, and characterization studies. A versatile, high‑quality reagent for molecular biology and protein engineering applications.
STEMCELL Technologies EasySep™ Mouse Epithelial Cell Enrichment Kit II, For processing: 1 x 10^9 cells
The EasySep™ Mouse Epithelial Cell Enrichment Kit II is designed to isolate epithelial cells from freshly dissociated mouse mammary tissues by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted non-epithelial cells with biotinylated antibodies and magnetic particles. The magnetically labeled cells are separated from untouched desired cells by using an EasySep™ magnet and simply pouring the desired cells into a new tube.This product replaces the EasySep™ Epithelial Cell Enrichment Kit (Catalog #19758) for even faster isolations. It also replaces the cell enrichment portion of the EasySep™ Mouse Mammary Stem Cell Enrichment Kit (Catalog #19757). Additionally, Anti-Mouse CD24 Antibody, Clone M1/69, PE (Catalog #60099PE) and Anti-Mouse CD49f Antibody, Clone GoH3, FITC (Catalog #60037FI) are required to complete the kit.
Perkin Elmer US LLC Low Flow Cold Trap Tube for ATD 400
Includes the following pieces: 1 Low Flow Trap Tube (L4271298) 6 Trap Packing Discs (L4271290)
STEMCELL Technologies EasySep™ RBC Depletion Reagent for RoboSep, For processing: 100 mL whole blood processed
The EasySep™ RBC Depletion Reagent is designed to immunomagnetically deplete red blood cells (RBCs) from fresh human peripheral blood, umbilical cord blood, bone marrow or leukapheresis samples by negative selection. RBCs are targeted with Tetrameric Antibody Complexes recognizing Glycophorin A and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Unwanted cells remain in the tube while desired cells are poured off.This product replaces the EasySep™ Human Glycophorin A Depletion Kit (Catalog #18352) for even faster cell isolations.
STEMCELL Technologies RoboSep™ Mouse PE Positive Selection Kit II, For processing: 5 x 10^9 cells
The EasySep™ Mouse PE Positive Selection Kit II is designed to isolate cells that are labeled with PE-conjugated antibodies. Desired cells are targeted with antibody complexes recognizing PE and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This kit replaces the EasySep™ Mouse PE Positive Selection Kit (Catalog #18554).For highly purified particle-free cells, we recommend the EasySep™ Release Mouse PE Positive Selection Kit (Catalog #17656). (https://www.stemcell.com/products/easysep-release-mouse-pe-positive-selection-kit.html)